The shrine lists its hours as Monday to Friday in the morning until mid-afternoon and Saturday and Sunday in the morning until early evening, with the exhibit and gift shop open the same hours. Mass is at noon from Monday to Saturday, and on Sunday in Spanish in the late morning and in English in the early afternoon. Confessions are heard Monday to Saturday in the late morning, and on Sunday around midday in Spanish and in the mid-afternoon in English. The Chaplet of Divine Mercy is prayed in the mid-afternoon every day, online only on Mondays. Parking for cars and buses is free, and the building is accessible to wheelchair users. These details were read from the shrine's site on October 10, 2026.

The blood relic was a gift of Cardinal Stanisław Dziwisz, John Paul II's longtime secretary and later Archbishop of Kraków. The shrine notes that pilgrims traditionally venerate a relic by touching or kissing the reliquary while asking the saint's intercession; veneration honors the saint and is not worship.

Good to know before you go: the Basilica of the National Shrine of the Immaculate Conception is close by, along the same Harewood Road, so the two are easily visited on one day. Another relic of his blood has been enshrined since 2012 at the National Shrine of The Divine Mercy in Stockbridge, Massachusetts. In recent years the Washington shrine has held a walking pilgrimage through the city for his October 22 memorial; ask the shrine whether and how it is held in a given year.